anti α tubulin antibody  (Cell Signaling Technology Inc)

Journal: International journal of molecular sciences

Article Title: Repeated Administrations of Polyphenolic Extracts Prevent Chronic Reflexive and Non-Reflexive Neuropathic Pain Responses by Modulating Gliosis and CCL2-CCR2/CX3CL1-CX3CR1 Signaling in Spinal Cord-Injured Female Mice.

doi: 10.3390/ijms26073325

Figure Lengend Snippet: Figure 6. Expression of GFAP, IBA1, CCL2, CCR2, CX3CL1, CX3CR1 and CatS in dPAG at the chronic phase of SCI-induced neuropathic pain after GSE15 and CE10 treatments in the first, third and sixth week post-injury. Protein expression was normalized to a-tubulin. Data are expressed as a relative percentage with respect to sham group (mean ± SEM). a–d: Groups not sharing a letter showed significant differences, p < 0.05. Experimental groups: sham (GFAP n = 6; IBA1 n = 6; CCL2 n = 6; CCR2 n = 7; CX3CL1 n = 6; CX3CR1 n = 6; CatS n = 6), SCI + saline (GFAP n = 6; IBA1 n = 6; CCL2 n = 6; CCR2 n = 7; CX3CL1 n = 6; CX3CR1 n = 6; CatS n = 6), SCI + GSE15 (GFAP n = 6; IBA1 n = 6; CCL2 n = 6; CCR2 n = 7; CX3CL1 n = 6; CX3CR1 n = 6; CatS n = 6), SCI + CE10 (GFAP n = 6; IBA1 n = 6; CCL2 n = 6; CCR2 n = 7; CX3CL1 n = 6; CX3CR1 n = 6; CatS n = 6). Figure S1 of Supporting Information file shows full-length blot images corresponding to the cropped Western blot presented in the figure.

Article Snippet: 2025, 26, 3325 24 of 30 Anti-α-tubulin antibody (1:1000, Cell Signaling, Danvers, MA, USA) was used as a loading control.

Techniques: Expressing, Saline, Western Blot

Journal: International journal of molecular sciences

Article Title: Repeated Administrations of Polyphenolic Extracts Prevent Chronic Reflexive and Non-Reflexive Neuropathic Pain Responses by Modulating Gliosis and CCL2-CCR2/CX3CL1-CX3CR1 Signaling in Spinal Cord-Injured Female Mice.

doi: 10.3390/ijms26073325

Figure Lengend Snippet: Figure 7. Expression of GFAP, IBA1, CCL2, CCR2, CX3CL1, CX3CR1 and CatS in vPAG at the chronic phase of SCI-induced neuropathic pain after GSE15 and CE10 treatments in the first, third and sixth week post-injury. Protein expression was normalized to a-tubulin. Data are expressed as a relative percentage with respect to sham group (mean ± SEM). a–d: Groups not sharing a letter showed significant differences, p < 0.05. Experimental groups: sham (GFAP n = 6; IBA1 n = 6; CCL2 n = 6; CCR2 n = 7; CX3CL1 n = 6; CX3CR1 n = 6; CatS n = 6), SCI + saline (GFAP n = 6; IBA1 n = 6; CCL2 n = 6; CCR2 n = 7; CX3CL1 n = 6; CX3CR1 n = 6; CatS n = 6), SCI + GSE15 (GFAP n = 6; IBA1 n = 6; CCL2 n = 6; CCR2 n = 7; CX3CL1 n = 6; CX3CR1 n = 6; CatS n = 6), SCI + CE10 (GFAP n = 6; IBA1 n = 6; CCL2 n = 6; CCR2 n = 7; CX3CL1 n = 6; CX3CR1 n = 6; CatS n = 6). Figure S1 of Supporting Information file shows full-length blot images corresponding to the cropped Western blot presented in the figure.

Article Snippet: 2025, 26, 3325 24 of 30 Anti-α-tubulin antibody (1:1000, Cell Signaling, Danvers, MA, USA) was used as a loading control.

Techniques: Expressing, Saline, Western Blot

Journal: International journal of molecular sciences

Article Title: Repeated Administrations of Polyphenolic Extracts Prevent Chronic Reflexive and Non-Reflexive Neuropathic Pain Responses by Modulating Gliosis and CCL2-CCR2/CX3CL1-CX3CR1 Signaling in Spinal Cord-Injured Female Mice.

doi: 10.3390/ijms26073325

Figure Lengend Snippet: Figure 8. Expression of GFAP, IBA1, CCL2, CCR2, CX3CL1, CX3CR1 and CatS in RVM at the chronic phase of SCI-induced neuropathic pain after GSE15 and CE10 treatments in the first, third and sixth week post-injury. Protein expression was normalized to a-tubulin. Data are expressed as a relative percentage with respect to sham group (mean ± SEM). a–d: Groups not sharing a letter showed significant differences, p < 0.05. Experimental groups: sham (GFAP n = 6; IBA1 n = 6; CCL2 n = 6; CCR2 n = 7; CX3CL1 n = 6; CX3CR1 n = 6; CatS n = 6), SCI + saline (GFAP n = 6; IBA1 n = 6; CCL2 n = 6; CCR2 n = 7; CX3CL1 n = 6; CX3CR1 n = 6; CatS n = 6), SCI + GSE15 (GFAP n = 6; IBA1 n = 6; CCL2 n = 6; CCR2 n = 7; CX3CL1 n = 6; CX3CR1 n = 6; CatS n = 6), SCI + CE10 (GFAP n = 6; IBA1 n = 6; CCL2 n = 6; CCR2 n = 7; CX3CL1 n = 6; CX3CR1 n = 6; CatS n = 6). Figure S2 of Supporting Information file shows full-length blot images corresponding to the cropped Western blot presented in the figure.

Article Snippet: 2025, 26, 3325 24 of 30 Anti-α-tubulin antibody (1:1000, Cell Signaling, Danvers, MA, USA) was used as a loading control.

Techniques: Expressing, Saline, Western Blot

Journal: International journal of molecular sciences

Article Title: Repeated Administrations of Polyphenolic Extracts Prevent Chronic Reflexive and Non-Reflexive Neuropathic Pain Responses by Modulating Gliosis and CCL2-CCR2/CX3CL1-CX3CR1 Signaling in Spinal Cord-Injured Female Mice.

doi: 10.3390/ijms26073325

Figure Lengend Snippet: Figure 9. Expression of GFAP, IBA1, CCL2, CCR2, CX3CL1, CX3CR1 and CatS in ACC at the chronic phase of SCI-induced neuropathic pain after GSE15 and CE10 treatments in the first, third and sixth week post-injury. Protein expression was normalized to a-tubulin. Data are expressed as a relative percentage with respect to sham group (mean ± SEM). a–d: Groups not sharing a letter showed significant differences, p < 0.05. Experimental groups: sham (GFAP n = 6; IBA1 n = 6; CCL2 n = 6; CCR2 n = 7; CX3CL1 n = 6; CX3CR1 n = 6; CatS n = 6), SCI + saline (GFAP n = 6; IBA1 n = 6; CCL2 n = 6; CCR2 n = 7; CX3CL1 n = 6; CX3CR1 n = 6; CatS n = 6), SCI + GSE15 (GFAP n = 6; IBA1 n = 6; CCL2 n = 6; CCR2 n = 7; CX3CL1 n = 6; CX3CR1 n = 6; CatS n = 6), SCI + CE10 (GFAP n = 6; IBA1 n = 6; CCL2 n = 6; CCR2 n = 7; CX3CL1 n = 6; CX3CR1 n = 6; CatS n = 6). Figure S3 of Supporting Information file shows full-length blot images corresponding to the cropped Western blot presented in the figure.

Article Snippet: 2025, 26, 3325 24 of 30 Anti-α-tubulin antibody (1:1000, Cell Signaling, Danvers, MA, USA) was used as a loading control.

Techniques: Expressing, Saline, Western Blot

Journal: International journal of molecular sciences

Article Title: Repeated Administrations of Polyphenolic Extracts Prevent Chronic Reflexive and Non-Reflexive Neuropathic Pain Responses by Modulating Gliosis and CCL2-CCR2/CX3CL1-CX3CR1 Signaling in Spinal Cord-Injured Female Mice.

doi: 10.3390/ijms26073325

Figure Lengend Snippet: Figure 10. Expression of GFAP, IBA1, CCL2, CCR2, CX3CL1, CX3CR1 and CatS in AMG at the chronic phase of SCI-induced neuropathic pain after GSE15 and CE10 treatments in the first, third and sixth week post-injury. Protein expression was normalized to a-tubulin. Data are expressed as a relative percentage with respect to sham group (mean ± SEM). a–d: Groups not sharing a letter showed significant differences, p < 0.05. Experimental groups: sham (GFAP n = 6; IBA1 n = 6; CCL2 n = 6; CCR2 n = 7; CX3CL1 n = 6; CX3CR1 n = 6; CatS n = 6), SCI + saline (GFAP n = 6; IBA1 n = 6; CCL2 n = 6; CCR2 n = 7; CX3CL1 n = 6; CX3CR1 n = 6; CatS n = 6), SCI + GSE15 (GFAP n = 6; IBA1 n = 6; CCL2 n = 6; CCR2 n = 7; CX3CL1 n = 6; CX3CR1 n = 6; CatS n = 6), SCI + CE10 (GFAP n = 6; IBA1 n = 6; CCL2 n = 6; CCR2 n = 7; CX3CL1 n = 6; CX3CR1 n = 6; CatS n = 6). Figure S4 of Supporting Information file shows full-length blot images corresponding to the cropped Western blot presented in the figure.

Article Snippet: 2025, 26, 3325 24 of 30 Anti-α-tubulin antibody (1:1000, Cell Signaling, Danvers, MA, USA) was used as a loading control.

Techniques: Expressing, Saline, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Disabled 1 Is Part of a Signaling Pathway Activated by Epidermal Growth Factor Receptor

doi: 10.3390/ijms22041745

Figure Lengend Snippet: The following antibodies were used in this study at the indicated dilutions.

Article Snippet: Phospho-Dab1 (Tyr232) Antibody , #3325 , Cell Signaling Technology (Danvers, MA, USA) , WB 1:500.

Techniques: Produced

Journal: Frontiers in Molecular Neuroscience

Article Title: Differential Action of Reelin on Oligomerization of ApoER2 and VLDL Receptor in HEK293 Cells Assessed by Time-Resolved Anisotropy and Fluorescence Lifetime Imaging Microscopy

doi: 10.3389/fnmol.2019.00053

Figure Lengend Snippet: The following antibodies were used in this study at the indicated dilutions.

Article Snippet: Phospho-Dab1 (Tyr232) , #3325 , Cell Signaling , WB 1:1,000.

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Synergistic Activity of Colistin plus Rifampin against Colistin-Resistant KPC-Producing Klebsiella pneumoniae

doi: 10.1128/AAC.00179-13

Figure Lengend Snippet: Synergistic bacteriostatic activities of different antimicrobial combinations against 10 colistin-resistant KPC-producing Klebsiella pneumoniae isolates

Article Snippet: Antagonism was not observed with any antibiotic combination ( ). table ft1 table-wrap mode="anchored" t5 caption a7 Isolate ST ΣFIC a of b : CST-RIF MER-GEN CST-GEN CST-MER CST-IMI CST-TIG IMI-GEN GEN-TIG MER-TIG IMI-TIG 2018/12 ST512 0.09 0.08 0.07 0.13 0.26 2.00 0.07 2.00 2.00 2.00 2604/12 ST512 0.38 0.38 0.07 1.13 0.25 2.00 0.38 0.56 2.00 2.00 2550/12 ST512 0.08 0.63 0.07 0.16 0.38 0.13 0.13 0.63 2.00 0.56 2762/12 ST512 0.09 1.00 0.75 2.00 1.13 0.75 0.56 0.56 2.00 2.00 3031/12 ST512 0.16 0.25 0.25 2.00 2.00 0.38 2.00 1.00 2.00 2.00 3177/12 ST512 0.09 0.50 0.53 0.50 2.00 1.00 0.75 2.00 2.00 1.00 3325/12 ST512 0.28 0.16 0.19 2.00 0.63 0.31 4.00 0.63 1.00 2.00 3342/12 ST512 0.09 0.63 1.00 2.00 2.00 0.63 2.00 1.00 2.00 1.00 3434/12 ST512 0.19 0.75 0.56 1.00 3.00 0.75 2.00 2.00 2.00 2.00 3515/12 ST512 0.06 0.75 0.52 0.50 0.19 0.26 0.56 0.75 2.00 2.00 2/12 ST101 0.08 2.00 0.56 2.00 2.00 0.50 2.00 2.00 2.00 2.00 4306/11 ST101 0.12 0.75 0.53 0.50 0.50 0.56 0.56 2.00 2.00 2.00 101/11 ST101 0.09 2.00 0.75 2.00 2.00 2.00 2.00 2.00 2.00 2.00 No. (% c ) with synergism 13 (100) 5 (38.5) 5 (38.5) 5 (38.5) 5 (38.5) 5 (38.5) 3 (23.1) 0 (0) 0 (0) 0 (0) Open in a separate window a ∑FIC = FIC-A + FIC-B (FIC, fractional inhibitory concentration; FIC-A, MIC of agent A in the presence of agent B divided by MIC of agent A alone; FIC-B, MIC of agent B in the presence of agent A divided by MIC of agent B alone). b Shading indicates synergism.

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Synergistic Activity of Colistin plus Rifampin against Colistin-Resistant KPC-Producing Klebsiella pneumoniae

doi: 10.1128/AAC.00179-13

Figure Lengend Snippet: Synergistic bactericidal activities of different antimicrobial combinations against 10 colistin-resistant KPC-producing Klebsiella pneumoniae isolates

Article Snippet: Antagonism was not observed with any antibiotic combination ( ). table ft1 table-wrap mode="anchored" t5 caption a7 Isolate ST ΣFIC a of b : CST-RIF MER-GEN CST-GEN CST-MER CST-IMI CST-TIG IMI-GEN GEN-TIG MER-TIG IMI-TIG 2018/12 ST512 0.09 0.08 0.07 0.13 0.26 2.00 0.07 2.00 2.00 2.00 2604/12 ST512 0.38 0.38 0.07 1.13 0.25 2.00 0.38 0.56 2.00 2.00 2550/12 ST512 0.08 0.63 0.07 0.16 0.38 0.13 0.13 0.63 2.00 0.56 2762/12 ST512 0.09 1.00 0.75 2.00 1.13 0.75 0.56 0.56 2.00 2.00 3031/12 ST512 0.16 0.25 0.25 2.00 2.00 0.38 2.00 1.00 2.00 2.00 3177/12 ST512 0.09 0.50 0.53 0.50 2.00 1.00 0.75 2.00 2.00 1.00 3325/12 ST512 0.28 0.16 0.19 2.00 0.63 0.31 4.00 0.63 1.00 2.00 3342/12 ST512 0.09 0.63 1.00 2.00 2.00 0.63 2.00 1.00 2.00 1.00 3434/12 ST512 0.19 0.75 0.56 1.00 3.00 0.75 2.00 2.00 2.00 2.00 3515/12 ST512 0.06 0.75 0.52 0.50 0.19 0.26 0.56 0.75 2.00 2.00 2/12 ST101 0.08 2.00 0.56 2.00 2.00 0.50 2.00 2.00 2.00 2.00 4306/11 ST101 0.12 0.75 0.53 0.50 0.50 0.56 0.56 2.00 2.00 2.00 101/11 ST101 0.09 2.00 0.75 2.00 2.00 2.00 2.00 2.00 2.00 2.00 No. (% c ) with synergism 13 (100) 5 (38.5) 5 (38.5) 5 (38.5) 5 (38.5) 5 (38.5) 3 (23.1) 0 (0) 0 (0) 0 (0) Open in a separate window a ∑FIC = FIC-A + FIC-B (FIC, fractional inhibitory concentration; FIC-A, MIC of agent A in the presence of agent B divided by MIC of agent A alone; FIC-B, MIC of agent B in the presence of agent A divided by MIC of agent B alone). b Shading indicates synergism.

Techniques: